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Islamabad closed one of the two border crossing for U.S. supplies for 10 days to protest that incident.

There was no indication of how long Islamabad could keep the border closed this time.

On Sunday, about 300 trucks carrying supplies to U.S.-led forces in Afghanistan were backed up at the Torkham border crossing in the northwest Khyber tribal area, the same crossing that was closed last year, as well as Chaman in southwestern Baluchistan province

Militants inside Pakistan periodically attack the slow-moving convoys and took advantage last year when the trucks were waiting for days to enter Afghanistan, torching 150.

“We are worried,” said driver Saeed Khan, speaking Sunday by telephone from the border terminal in Torkham. “This area is always vulnerable to attacks. Sometimes rockets are lobbed at us. Sometimes we are targeted by bombs.”

Some drivers said paramilitary troops had been deployed to protect their convoys since the closures, but others were left without any additional protection. Even those who did receive troops did not feel safe.

“If there is an attack, what can five or six troops do?” said Niamatullah Khan, a fuel truck driver who was parked with 35 other vehicles at a restaurant about 125 miles from Chaman.

NATO ships nearly 50 percent of its nonlethal supplies such as fuel, food and clothes to its troops in Afghanistan through Pakistan. Critical supplies such as ammunition are airlifted directly to Afghan air bases.

A NATO official closely involved with the Afghan war said there likely will be no immediate negative effect from Pakistan’s decision to close its border crossings. NATO has built up a large stockpile of military and other supplies that could enable operations to continue at their current level for several months, said the official, speaking on condition of anonymity because of the sensitivity of the matter.

NATO has reduced the nonlethal supplies it ships through Pakistan from a high of about 80 percent by using routes through Central Asia. The northern logistics link could be expanded to make up for the Pakistani closure, but it would leave NATO heavily dependent on Russia at a time when ties with Moscow are increasingly strained.

In addition to closing its border crossings, Pakistan gave the U.S. 15 days to vacate Shamsi Air Base in Baluchistan. Washington uses the base to service drones targeting al Qaeda and Taliban militants in Pakistan’s tribal region when they cannot return to their bases inside Afghanistan because of weather conditions or mechanical difficulty, U.S. and Pakistani officials said. They spoke on condition of anonymity to discuss sensitive matters.

The drone strikes are very unpopular in Pakistan, and Pakistani military and civilian leaders say publicly that the U.S. carries them out without their permission. But privately, they allow them to go on, and even help with targeting for some of them.

Rahim Faiez reported from Kabul, Afghanistan. Associated Press writers Riaz Khan in Peshawar, Pakistan; Abdul Sattar in Quetta, Pakistan; Matiullah Achakzai in Chaman, Pakistan; Deb Riechmann in Kabul; Anne Flaherty in Washington; and Slobodan Lekic in Brussels contributed to this report.