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“I called myself to cancel my advertising. I got a busy signal,” he deadpanned at the start of Monday’s program.

Yet he appeared defiant and suggested he would have little trouble finding new sponsors.

“I reject millions of dollars of advertisers a year, much to the chagrin of my hard-working sales staff,” Mr. Limbaugh said.

The tumult began last week when Mr. Limbaugh discounted Ms. Fluke’s appearance on Capitol Hill.

He said last Wednesday: “What does it say about the college coed … who goes before a congressional committee and essentially says that she must be paid to have sex? It makes her a slut, right? It makes her a prostitute. She wants to be paid to have sex.”

He dug in a day later, refusing to give ground.

“If we’re going to have to pay for this, then we want something in return, Ms. Fluke,” Mr. Limbaugh said. “And that would be the videos of all this sex posted online so we can see what we’re getting for our money.”

On Friday, still defiant even after Democrats beat back Republican challenges to the new health-care requirement, Mr. Limbaugh scoffed at the Democrats’ talk of a conservative “war on women.”

Mr. Obama, aware of the political advantages of branding all conservatives as supporters of Mr. Limbaugh’s views, telephoned Ms. Fluke from the Oval Office on Friday to offer his support.

A day later, Mr. Limbaugh apologized to Ms. Fluke.

Yet even on Monday, Mr. Limbaugh didn’t back away from his criticism of Ms. Fluke’s appearance on Capitol Hill. He questioned why she was invited to testify.

“She doesn’t have any expertise,” Mr. Limbaugh said during the second hour of his radio show.