Team captain Jim Courier made the announcement Thursday at the draw ceremony.
Isner has not played competitively since withdrawing from his opening-round match at the APIA International Sydney three weeks ago with a knee injury.
The Brazilian team will consist of singles players Thomaz Bellucci and Thiago Alves and the doubles team of Marcelo Melo and Bruno Soares. Querrey will play Bellucci in Friday’s opening match followed by the Isner-Alves match.
The three-day event begins Friday at the Jacksonville Veterans Memorial Arena.
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