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“There is a long history with these cases which included an inquiry from Terry McAuliffe to the Secretary of Homeland Security when USCIS denied an amendment for Gulf Coast Funds Management, LLC a few years back,” reads an internal e-mail obtained by Mr. Grassley’s office.

“According to the DHS OIG, its investigation revolves around allegations that Director Mayorkas allegedly assisted with the approval of Gulf Coast’s Regional Center application after it was denied by both the USCIS California Service Center and the USCIS Administrative Appeals Office,” Mr. Grassley wrote.

A McAuliffe spokesman on Tuesday issued a statement in response to reports that Homeland Security’s inspector general was investigating Mr. Mayorkas and his involvement securing a EB-5 visa on behalf of Gulf Coast.

“The investigation does not involve Terry and we hope that it is completed in a timely matter,” Josh Schwerin said.

In letters to Mr. Rodham and GreenTech CEO Charles Wang last week, Mr. Grassley asked for information to be provided by July 24, including a copy of Gulf Coast’s narrative and business plan provided to USCIS and the total amount of foreign capital invested with Gulf Coast.

Gulf Coast, founded in 2008 and described by officials as a federally approved EB-5 regional center that supports job-creation programs in Louisiana and Mississippi, issued a statement of its own Tuesday when the investigation was first reported.

“Our management abides by all regulations under USCIS, and [Gulf Coast’s] contact with USCIS has been limited to procedural inquiries. [Gulf Coast] has not sought assistance from USCIS to resolve a rejected appeal. In fact, we are not aware of any investor visa applications associated with our Regional Center being denied. [Gulf Coast] is not part of any investigation by Department of Homeland Security,” the company said in a statement Tuesday.