The Yankees captain, 0 for 9 in four games, hit into his third double play in the first and had a grounder to third during the third.
Jeter, who announced last month that he will be retiring after the 2014 season, broke his left ankle in the 2012 AL championship series and was limited to 17 games last year.
Jeter was the designated hitter Tuesday in his first back-to-back games this spring. He played shortstop in in a 4-2 win Monday over the Washington Nationals.
Jeter had already left the clubhouse before it was opened to reporters late in the game.
Orioles: Wei-Yin Chen allowed two runs and five hits, including Francisco Cervelli’s solo homer, over two innings in his first spring training start. He had right knee surgery during the offseason.
“I just feel comfortable right now,” Chen said through an interpreter.
Yankees: Fifth starter candidate David Phelps, who worked out of two-on, no-out jams in the first and second, gave up one run and five hits in 2 2-3 innings.
Orioles: Right-hander Edgmer Escalona has been shut down for three weeks due to right shoulder inflammation.
Outfielder Henry Urrutia (right shoulder) could return in a few days.
Yankees: Manager Joe Girardi remains optimistic that first baseman Mark Teixeira (wrist) and outfielder/designated hitter Alfonso Soriano (flu-like symptoms) will both play in their first spring training game Thursday against Philadelphia.
Shortstop Brendan Ryan played with a bruised left hand. He got hit by a liner during batting practice.