- The Washington Times - Tuesday, October 22, 2002


In a step toward creating herds of pigs that could provide organs for transplanting into humans, Italian researchers manipulated swine sperm to make an animal strain that carries human genes in the heart, liver and kidneys.

Researchers at the University of Milan mixed swine sperm with the DNA of a human gene called decay accelerating factor, or DAF, and then used the modified sperm to fertilize pig eggs.

The eggs were put into sows to produce litters of pigs that carried the human gene. DAF has been shown to help overcome rejection of pig organs in nonhuman primates.

"What we obtain at high efficiency and low cost is genetically modified pigs expressing the human protein," said Marialuisa Lavitrano, a Milan researcher and first author of the study appearing today in the Proceedings of the National Academy of Sciences.

Miss Lavitrano said that 205 piglets in 20 litters were produced using the modified sperm technique and the human genes were present in 20 percent to 50 percent of the young. Tests showed that the human genes were present in the animals' central organs and that the human genes would be passed along to later generations of pigs.

Organs from the test animals are not ready for transplantation into humans because there are still pig genes that would cause the organs to be quickly rejected, Miss Lavitrano said.

But she said the technique shows that by adding human genes to pig sperm it is possible to develop animals with organs that will not be rejected by the human immune system.

"They could be the starting point for new [gene transfer] experiments," she said.

Miss Lavitrano said that five to seven other pig genes will need to be silenced or replaced by human genes before useful organs could be harvested from the animals.

"With our efficiency we think we can add the other genes and breed the animals in about two years," she said.

Medical scientists have been working to change swine genes so that modified animals could be used to make organs that would be tolerated by the human body. The goal is to create a special strain of pigs that could make organs that could be used to replace ailing livers, kidneys and hearts in humans.

It is hoped that the use of pig organs would relieve the shortage of human organs available for transplant. It has been estimated that about 4,000 people die each year while awaiting donor organs.

Pig parts have been used to replace heart valves in humans, but replacing whole organs represents a significantly more complex challenge.

One of the concerns about such transplants is the chance that unrecognized swine viruses could be transferred into human patients along with the transplanted organ, experts say.

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