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“We are always extremely disappointed to learn of potential links between players and the use of performance-enhancing substances,” MLB said in a statement. “Only law enforcement officials have the capacity to reach those outside the game who are involved in the distribution of illegal performance-enhancing drugs. … We are in the midst of an active investigation and are gathering and reviewing information.”

A baseball official, speaking on condition of anonymity because he was not authorized to make public statements, said Monday that MLB did not have any documentation regarding the allegations. If MLB does obtain evidence, the players could be subject to discipline. First offenses result in a 50-game suspension and second infractions in 100-game penalties. A third violation results in a lifetime ban.

Rodriguez is sidelined for at least the first half of the season after hip surgery Jan. 16. A 50-game suspension would cost him $7.65 million of his $28 million salary.

“The news report about a purported relationship between Alex Rodriguez and Anthony Bosch are not true,” Rodriguez said in a statement issued by a publicist. “He was not Mr. Bosch’s patient, he was never treated by him and he was never advised by him. The purported documents referenced in the story _ at least as they relate to Alex Rodriguez _ are not legitimate.”

Jay Reisinger, a lawyer who has represented Rodriguez in recent years, said the three-time AL MVP had retained Roy Black, an attorney from Rodriguez’s hometown of Miami. Black’s clients have included Rush Limbaugh and William Kennedy Smith.

Bosch did not return a phone message seeking comment.

MLB hopes to gain the cooperation of Bosch and others connected with the clinic, another baseball official said, also on condition of anonymity because no public statements on the matter were authorized. In order to successfully discipline players based on the records, witnesses would be needed to authenticate them, the official said.

Players could be asked to appear before MLB for interviews, but the official said MLB would be reluctant to request interviews before it has more evidence.

Rodriguez spent years denying he used PEDs before Sports Illustrated reported in February 2009 that he tested positive for two steroids in MLB’s anonymous survey while with the Texas Rangers in 2003. Two days later, he admitted in an ESPN interview that he used PEDs over a three-year period. He has denied using PEDs after 2003.

If the new allegations were true, the Yankees would face high hurdles to get out of the final five years and $114 million of Rodriguez’s record $275 million, 10-year contract. Because management and the players’ union have a joint drug agreement, an arbitrator could determine that any action taken by the team amounted to multiple punishments for the same offense.

But if Rodriguez were to end his career because of the injury, about 85 percent of the money owed by the Yankees would be covered by insurance, one of the baseball officials said.

The Yankees said “this matter is now in the hands of the commissioner’s office” and said they will not comment further until MLB’s investigation ends.

Gonzalez, 21-8 for the Washington Nationals last season, posted on his Twitter feed: “I’ve never used performance enhancing drugs of any kind and I never will, I’ve never met or spoken with tony Bosch or used any substance provided by him. anything said to the contrary is a lie.”

Colon was not issuing a statement, agent Adam Katz said through spokeswoman Lisa Cohen.

“We are aware of certain allegations and inferences,” Cruz’s law firm, Farrell & Reisinger, said in a statement. “To the extent these allegations and inferences refer to Nelson, they are denied.”

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